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Abstract            Volume:11  Issue-5  Year-2023         Original Research Articles

Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Antibiotic Property of Edible and Non-Edible Wild Mushrooms from Menengai Crater in Kenya
Paul Njenga Waithaka1* and Githaiga Benson Muriuki2
1School of Pure and Applied Sciences, Kirinyaga University, P.O. Box, 143, Kerugoya
2Department of Biological Sciences Egerton University, P. O. Box, 536, Njoro, Kenya
*Corresponding author

Antibiotic resistance is a global challenge of our times. To avert the problem, search for alternative antibiotics from natural sources is inevitable. This study sought to extract antibiotics from edible and non-edible wild mushrooms from Menengai crater. The mushroom samples were collected in new khaki bags from Menengai crater and transported to the laboratory. The samples were dried under room temperature (25±2oC). Extraction of the crude extracts was carried out using ethyl acetate, methanol, ethanol and acetone. Antibiotic bioassay was done using Kirby Bauer disc diffusion technique against Enterococcus faecalis (ATCC 29212), Staphilococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 70063), Aspergillus flavus (ATCC 9170), Aspergillus fumigates (96918) and Candida albicans (10231). The yield of crude extract obtained using ethyl acetate, methanol, ethanol and acetone varied significantly (P=0.009). Likewise, the total phenolic content varied significantly among the extracts (P= 0.000105). However, the zones of inhibition did not vary significantly among the bacterial (P=0.98) and fungal pathogens (P=0.61). The selected mushroom samples produced bioactive compounds that inhibited growth of the selected pathogenic microorganisms. There is need to characterize the active compounds in the crude extracts obtained from the mushroom samples.

Keywords: Antibiotic, Extracts, Menengai, Mushroom, Solvents, fatty acid content.
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How to cite this article:

Paul Njenga Waithaka and Githaiga Benson Muriuki. 2023. Antibiotic Property of Edible and Non-Edible Wild Mushrooms from Menengai Crater in KenyaInt.J.Curr.Res.Aca.Rev. 11(5): 8-14
doi: https://doi.org/10.20546/ijcrar.2023.1105.002
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.