Abstract            Volume:11  Issue-5  Year-2023         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Antibiotic resistance is a global challenge of our times. To avert the problem, search for alternative antibiotics from natural sources is inevitable. This study sought to extract antibiotics from edible and non-edible wild mushrooms from Menengai crater. The mushroom samples were collected in new khaki bags from Menengai crater and transported to the laboratory. The samples were dried under room temperature (25±2^oC). Extraction of the crude extracts was carried out using ethyl acetate, methanol, ethanol and acetone. Antibiotic bioassay was done using Kirby Bauer disc diffusion technique against Enterococcus faecalis (ATCC 29212), Staphilococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 70063), Aspergillus flavus (ATCC 9170), Aspergillus fumigates (96918) and Candida albicans (10231). The yield of crude extract obtained using ethyl acetate, methanol, ethanol and acetone varied significantly (P=0.009). Likewise, the total phenolic content varied significantly among the extracts (P= 0.000105). However, the zones of inhibition did not vary significantly among the bacterial (P=0.98) and fungal pathogens (P=0.61). The selected mushroom samples produced bioactive compounds that inhibited growth of the selected pathogenic microorganisms. There is need to characterize the active compounds in the crude extracts obtained from the mushroom samples.

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