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Abstract            Volume:11  Issue-5  Year-2023         Original Research Articles

Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Morphological and Phenotypic Characteristics of Phosphate Solubilizing Bacteria, Isolated from Acidic soils of Tigray, Ethiopia
Aklil Gebremedhin1* and Haile Alene2
1Tigrai Agricultural Research Institute, Mekelle Soil Research center, Soil Microbiology Researcher
2Tigrai Agricultural Research Institute, Humera Agricultural Research Center, Soil Fertility Researcher
*Corresponding author

Phosphate solubilise bacteria (PSB) has the ability to convert insoluble phosphates in to soluble phosphates. Characterization of this bacterium as inoculants in acidic soils increases its phosphorus uptake by the respective plant as well as its productivity. This research was conducted on low pH soils (<6.00) of Tsegede area, located in Western zone of Tigray region. The study revealed that a series of morphological and biochemical test were conducted, all the isolated phosphate solubilize bacteria were indicated gram negative, rod shaped and mucous producing. All of the strains were grew well at pH 6 and 7, temperature 15-35 oC and at 4% salt concentration. A positive response of the tested isolates was shown for 1.5 mg/l and 3.0 mg/l Mn SO4 and 0.25 mg/l Zn SO4. The anticipated significant response of antibiotic resistance was recorded. Hundred percent of the isolates were utilize L-arginine as N source as well as D-glucose, D-fructose and Lactose as of carbon sources. The highest solubilization index (SI) was observed from isolate ESB (2.21) followed by ESE (2.18) and the smallest SI was recorded from CHB, ITA and ESC (1.67). The highest colony diameter was obtained from Chegargudo at soil pH of 5.30 and from Endaslasea at soil pH of 4.92. The lowest colony diameter (4.00mm) was found at soil pH of 5.70 and 5.76. The tested phosphate solubilize bacteria had also a significant response after they grow on PVC broth media, accordingly the highest PS was obtained from FSA (30.70 ESB mg L-1) followed by ITA (29.96 mg L-1). The potential of the collected PSB to release phosphate indicates that a drop down of pH values from7.0 to 4.9.

Keywords: phosphate solubilisation, antibiotics, Biochemical, Morphological, Pikovaskay’s.
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How to cite this article:

Aklil Gebremedhin and Haile Alene. 2023. Morphological and Phenotypic Characteristics of Phosphate Solubilizing Bacteria, Isolated from Acidic soils of Tigray, Ethiopia.Int.J.Curr.Res.Aca.Rev. 11(5): 38-47
doi: https://doi.org/10.20546/ijcrar.2023.1105.006
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.