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Abstract                 Volume:4  Issue-1  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
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Diagnostic Role for Antibodies against Heat Sock Protein 70 In Perilymphatic Fluid of Children with Cochlear Implant Surgery: A Case Control Study in Tehran, Iran
Mohammad Reza Shokrollahi1 , Mohammad Farhadi2 , Samileh Noorbakhsh3 *, Hesamodin Emam Jomeh2 and Azardokht Tabatabaei3
Qom University of Medical Sciences, Qom, Iran
ENT, Head & Neck Surgery Research Center, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
Research Center of Pediatric Infectious Diseases, Hazrat Rasul Hospital, Iran University of Medical Sciences, Tehran, Iran
*Corresponding author
Abstract:

Serum antibodies against HSP 60 and HSP 70 are diagnostic for infectious diseases. Goal of study: quantification the antibodies against Heat Sock Ptotein 70Ab (HSP 70) in serum and perilymphatic fluid of Children with cochlear implant surgery A cross sectional study had done upon 117 children undergoing cochlear implant surgery in the cochlear implant ward. Age of cases was between 6 months -14years ;mean :3. 4±3. 1years;. 58% male,42% female. Idiopathic SNHL diagnosed in 39. 4 % of cases. Blood samples (2 ml) were taken, then centrifuged and transferred to our research laboratory. During surgery period 0. 5-3 ml of Perilymphatic fluids were taken by ENT specialist in operation room. All samples were kept frozen and stored at -70°C. Hsp70 level determined by using the ELISA kits (STRESS XPRESS,stressgen biotechnologies Canada). The Heat Sock Ptotein70Ab (HSP 70) level determined in serum and perilymphatic fluid SNHL in cochlear implanted children, and compared between idiopathic type of SNHL and hereditory type. Pvalue <0. 05 was considered significant Findings: One hundered twenty four cases enrolled in this study. ; 58% male,42% female. Age of cases was between 6 months - 14years ;mean :3. 4±3. 1years;. Idiopathic SNHL diagnosed in 39. 4 % of cases ( Fig- 1) The Heat Sock Ptotein70 Ab detected in sera of 46. 8% of studied cases. Heat Sock Ptotein70 Ab level in perilymphatic fluid was higher in idiopathic SNHL cases (P = 0. 000). A ROC curve analysis showed an AUC(area under ROC curve) 0. 731 (95% CI; 0. 629-0. 834, P = 0. 000). HSP70 Ab (Cutoff level 0. 39 pg/dl ) in perilymphatic fluid had 76% sensitivity and 60% specificity for differentiating the idiopathic type of SNHL from hereditory ones. . Assesment the HSP70 level in perilymphatic fluid (76% sensitivity and 60 specifity ) might be useful for differentiating the idiopathic type of SNHL from hereditory ones. This test can potentially assist clinicians in faster diagnosis and specific treatment in cases with progressive idiopathic SNHL. Further studies are necessary in order to identify the immunologic role of the these antibodies and their impact on the prognosis of SNHL.

Keywords: Cochlear implant, SNHL, HSP70, Perilymphatic fluid
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How to cite this article:

Mohammad Reza Shokrollahi, Mohammad Farhadi, Samileh Noorbakhsh, Hesamodin Emam Jomeh and Azardokht Tabatabaei. 2016. Diagnostic Role for Antibodies against Heat Sock Protein 70 In Perilymphatic Fluid of Children with Cochlear Implant Surgery: A Case Control Study in Tehran, Iran. IInt.J.Curr.Res.Aca.Rev. 4(1): 184-191
doi: http://dx.doi.org/10.20546/ijcrar.2016.401.018