IJCRAR is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRAR Articles.

INDEXED IN INDEX COPERNICUS - ICI Journals Master List 2016 - IJCRAR--ICV 2016: 81.15 For more details click here

Abstract                 Volume:4  Issue-8  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Purification and Properties of β-lactamase from Escherichia coli and Klebsiella pneumonia
Husain F. Hassan*, Nada E. Abd-Al Wahab and Salah S. Zain Al-abdeen
Department of Biology, College of Science, University of Kirkuk, Iraq
*Corresponding author
Abstract:

β- lactamase was purified from a β-lactam antibiotic resistant strain of Escherichia coli and Klebsiella pneumoniae. The purified enzyme of Escherichia coli and Klebsiella pneumoniae by ammonium sulfate fractionation followed by Sephadex G75 gel filteration,resuled in an overall recovery of 30% with purification fold of 63 (E.coli) and of 23%with purification fold of 33(K.pnemoniae). The molecular weight of the native enzyme as determined by gel filteration was 32000 dalton (E.coli) and 35000 dalton (K.pneumoniae).The enzyme showed, a unique substrate profile by hydrolysing most of the Cephalosporines at a high rate.

Keywords: β-lactamase,Escherichia coliand Klebsiella pneumoniae
Download this article as Download

How to cite this article:

Husain F. Hassan, Nada E. Abd-Al Wahab and Salah S. Zain Al-abdeen. 2016. Purification and Properties of β-lactamase from Escherichia coli and Klebsiella pneumoniae.Int.J.Curr.Res.Aca.Rev. 4(8): 122-130
doi: http://dx.doi.org/10.20546/ijcrar.2016.408.011