Abstract Volume:4 Issue-8 Year-2016 Original Research Articles
|Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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β- lactamase was purified from a β-lactam antibiotic resistant strain of Escherichia coli and Klebsiella pneumoniae. The purified enzyme of Escherichia coli and Klebsiella pneumoniae by ammonium sulfate fractionation followed by Sephadex G75 gel filteration,resuled in an overall recovery of 30% with purification fold of 63 (E.coli) and of 23%with purification fold of 33(K.pnemoniae). The molecular weight of the native enzyme as determined by gel filteration was 32000 dalton (E.coli) and 35000 dalton (K.pneumoniae).The enzyme showed, a unique substrate profile by hydrolysing most of the Cephalosporines at a high rate.
How to cite this article:Husain F. Hassan, Nada E. Abd-Al Wahab and Salah S. Zain Al-abdeen. 2016. Purification and Properties of β-lactamase from Escherichia coli and Klebsiella pneumoniae.Int.J.Curr.Res.Aca.Rev. 4(8): 122-130