Abstract Volume:4 Issue-2 Year-2016 Original Research Articles
|Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : email@example.com
The study evaluated the effect of selected Vinca alkaloids on the microtubule network of H22 cells and the recovery of its disruption. Cells were treated with Vincristine at various concentrations from 2 μg/l to 800 μg/l for 60 min, or with Vincristine at a concentration of 4615 μg/l and 9230 μg/l for 60 min. Microtubules were detected by means of indirect immunofluorescence. The damage was examined in a fluorescence microscope. Also, cells were treated for 60 min with vinblastine at concentrations of 2 μg/l or 800 μg/l for 60 min, and the recovery process was studied in time intervals of 6, 7, 8, 9, 10 hours, or 12 hours, respectively. Differences in the arrangement of microtubules were assessed by means of a software for quantification of the cytoskeleton changes in cells treated with Vincristine at a concentration of 20 μg/l and in untreated control cells.
How to cite this article:Ibrahim Hadi Mohammed. 2016. Avinca alkaloid effect on microtubulues of H22 (Hepatic cell line).Int.J.Curr.Res.Aca.Rev. 4(2): 9-17