Abstract            Volume:5  Issue-2  Year-2017         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

This research was conducted to study the spread of an important unicellular protozoan Trichomonas gallinae isolated from the mouth and esophagus of three groups of domestic pigeon Colomba livia domestica by wet mount preparation. A total of 609 domestic pigeons were tested in Kirkuk city, Iraq from August 2015 to April 2016, the total infectious rate of the prevalence were 49.26%, distributed to 64.92%, 50.98% and 30.41% in Alzajel, pakistani and zubaeri pigeon respectively. The present study compared three different stains to characterize the shape of T. gallinae namely Giemz, Gram and for first time Field’s stain, the Giemza stain showed efficient to stain T. gallinae more than other two stains, although Field’s stain requires less time for staining compared to Giemza and Gram’s stain. The parasites are cultivated in three prepared culture media (In-tube culture system), which are modified Diamond media (TYM), Cysteine Peptone Maltose media (CPLM) and Trichomonas CM0161 media (CM 161) with the initial inoculation of 2X105 cell/ml, the fourth media was In-pouch culture system that is commercially available with the initial inoculation of 105 cell/ml. In-pouch TV culture media is used for the first time to diagnose and to grow T. gallinae which is a special media for diagnosis of the vaginal trichomoniasis caused by Trichomonas vaginalis. The result showed the best growth of T. gallinae  in In-pouch media compared with In-tube culture system, modified diamond media and CM 161 gave the high growth to 48 hours while CPLM gave the high growth from 24 hours, the CM 161 media showed acute decrease in the number of T. gallinae after 48 hour.

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