Warning: mysql_connect(): It is not safe to rely on the system's timezone settings. You are *required* to use the date.timezone setting or the date_default_timezone_set() function. In case you used any of those methods and you are still getting this warning, you most likely misspelled the timezone identifier. We selected the timezone 'UTC' for now, but please set date.timezone to select your timezone. in /home/ijcmasc1/public_html/ijcrar.com/IjcrarBackOffice/ConfigDB/DBCon.php on line 2

Deprecated: mysql_connect(): The mysql extension is deprecated and will be removed in the future: use mysqli or PDO instead in /home/ijcmasc1/public_html/ijcrar.com/IjcrarBackOffice/ConfigDB/DBCon.php on line 2
International Journal of Current Research and Academic Review-e-iSSN - 2347 - 3215

IJCRAR is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRAR Articles.

INDEXED IN INDEX COPERNICUS - ICI Journals Master List 2016 - IJCRAR--ICV 2016: 81.15 For more details click here

Abstract                 Volume:5  Issue-2  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq
Sadia Sh. Hamad* and Hazha H. Hassan
*Department of Parasitology, College of Science, University of Kirkuk, Iraq
*Corresponding author
Abstract:

This research was conducted to study the spread of an important unicellular protozoan Trichomonas gallinae isolated from the mouth and esophagus of three groups of domestic pigeon Colomba livia domestica by wet mount preparation. A total of 609 domestic pigeons were tested in Kirkuk city, Iraq from August 2015 to April 2016, the total infectious rate of the prevalence were 49.26%, distributed to 64.92%, 50.98% and 30.41% in Alzajel, pakistani and zubaeri pigeon respectively. The present study compared three different stains to characterize the shape of T. gallinae namely Giemz, Gram and for first time Field’s stain, the Giemza stain showed efficient to stain T. gallinae more than other two stains, although Field’s stain requires less time for staining compared to Giemza and Gram’s stain. The parasites are cultivated in three prepared culture media (In-tube culture system), which are modified Diamond media (TYM), Cysteine Peptone Maltose media (CPLM) and Trichomonas CM0161 media (CM 161) with the initial inoculation of 2X105 cell/ml, the fourth media was In-pouch culture system that is commercially available with the initial inoculation of 105 cell/ml. In-pouch TV culture media is used for the first time to diagnose and to grow T. gallinae which is a special media for diagnosis of the vaginal trichomoniasis caused by Trichomonas vaginalis. The result showed the best growth of T. gallinae  in In-pouch media compared with In-tube culture system, modified diamond media and CM 161 gave the high growth to 48 hours while CPLM gave the high growth from 24 hours, the CM 161 media showed acute decrease in the number of T. gallinae after 48 hour.

Keywords: Trichomonas gallinae, Domestic pigeon,Colomba livia domestica.
Download this article as Download

How to cite this article:

Sadia Sh. Hamad and Hazha H. Hassan. 2017. Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq.Int.J.Curr.Res.Aca.Rev. 5(2): 10-18
doi: http://dx.doi.org/10.20546/ijcrar.2017.502.002