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Abstract                 Volume:5  Issue-5  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Analysis of Wild Chickpea Seed Proteins for Lectin Composition
Ajay Kumar Gautam1, Nidhi Srivastava2, Amit Kumar Singh Chauhan3 and Sameer S.Bhagyawant1*
1School of Studies in Biotechnology, Jiwaji University, Gwalior, India
2Department of Bioscience and Biotechnology, Banasthali University, Banasthali, India
*Corresponding author
Abstract:

Lectins are carbohydrate-binding proteins of non-immunoglobulin nature and are ubiquitous in nature.  Study was undertaken with an objective to determine the lectin concentration in 15 wild chickpea seeds, which was unknown till date. Presence of lectin was assayed using human (A, B, AB and O) and rabbit erythrocytes, Trypsinized rabbit erythrocytes gave considerable lectin activity under in-vitro conditions. A titre was observed in 4-12 wells giving 8-4096 hemagglutination units (HAU). The specific activity was found highest in Cicer reticulatum ILWC-292 having 589.3 titre/mg. The lectins was stable for about 4-6 h and did not inhibit simple sugar moieties, but with complex sugars like desialated fetuin only. The primary role of lectin is to provide defence thus enhancing crop yield. Based on this screening data, ILWC-292 and ILWC-21 accessions may be used as parent in executing future breeding programme.

Keywords: Lectin, chickpea, hemagglutination.
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How to cite this article:

Ajay Kumar Gautam, Nidhi Srivastava, Amit Kumar Singh Chauhan and Sameer S. Bhagyawant. 2017. Analysis of Wild Chickpea for Lectin Composition.Int.J.Curr.Res.Aca.Rev. 5(5): 8-14
doi: https://doi.org/10.20546/ijcrar.2017.505.002