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Abstract                 Volume:5  Issue-6  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater
N.Benit and A. Stella Roslin*
Department of Botany, Holy Cross College, Nagercoil, Kanyakumari District, Tamilnadu 629 001, India
*Corresponding author
Abstract:

The aim of this study is to optimize extracellular polysaccharide (EPS) production from Pseudomonas aeruginosa B01 with potent antibacterial and larvicidal activity. EPS producing P. aeruginosa B01 was isolated from wastewater. Process parameters were optimized to enhance the production of EPS. EPS was fractionated with two-, three-volume of various solvent and antibacterial and larvicidal activities were evaluated. The bacterial cell growth was maximum after 48 h fermentation period, however, EPS production was maximum after 72 h. EPS production was found to be high when this organism was grown in the medium containing 1% maltose, 1% ammonium sulphate and 0.1% Mn2+ ions. EPS production was maximum at pH 7.5 and at 30 °C. Ethanol precipitated fractions showed high activity than other tested solvents. It was further subjected to Thin Layer Chromatography. EPS showed potent larvicidal activity against mosquito larva and showed potent activity against various pathogenic bacteria.

Keywords: Pseudomonas aeruginosa, Extracellular polysaccharide, Optimization, Antibacterial, Larvicidal.
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How to cite this article:

Benit, N. and Stella Roslin, A. 2017. Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater.Int.J.Curr.Res.Aca.Rev. 5(6): 6-15
doi: https://doi.org/10.20546/ijcrar.2017.506.002