Abstract            Volume:5  Issue-7  Year-2017         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

The antiproliferative effect of the purified L-Asparaginase enzyme on different tumor human cell lines have been investigated using human leukemic cells. These included MCF 7 (breast cancer cell line), MDA-MB435S (Breast Metastatic cancer), HeLa cell line, Human small cell lung cancer cell line H69PR (ATCC® CRL-11350™) and COLO 205 (ATCC® CCL-222™). The dose- and time-dependent antitumor and cytotoxic effects of L-asparaginase from Streptomyces enissocaesilis have been studied. We investigated the sensitivity of tumor cells towards L-asparaginase, as well the effect of L-asparaginase on cell growth rate, protein and DNA synthesis in the presence of various cytostatics. Cell cycle analysis by flow cytofluorimetry and detection of apoptotic cells before and after treatment with L-asparaginase were studied. Experiments have been conducted to investigate whether administration of L-asparaginase, which results in hydrolysis of extracellular asparagine, arrests asparagine dependent protein synthesis and causes subsequent inhibition of cell growth followed by decreased proliferation of leukemic cells.

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