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Abstract                 Volume:5  Issue-7  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Efficacy of the Antiproliferative Effect of L-Asparaginase from Streptomyces enissocaesilis on Different Tumor Cell Lines
B. Sirisha1* and R. Haritha2
1Department of Biotechnology, College of Science and Technology, Andhra University, Visakhapatnam 530 003, India
2Department of Biotechnology, Visakha Government Degree College (Women), Visakhapatnam 530 020, India
*Corresponding author
Abstract:

The antiproliferative effect of the purified L-Asparaginase enzyme on different tumor human cell lines have been investigated using human leukemic cells. These included MCF 7 (breast cancer cell line), MDA-MB435S (Breast Metastatic cancer), HeLa cell line, Human small cell lung cancer cell line H69PR (ATCC® CRL-11350™) and COLO 205 (ATCC® CCL-222™). The dose- and time-dependent antitumor and cytotoxic effects of L-asparaginase from Streptomyces enissocaesilis have been studied. We investigated the sensitivity of tumor cells towards L-asparaginase, as well the effect of L-asparaginase on cell growth rate, protein and DNA synthesis in the presence of various cytostatics. Cell cycle analysis by flow cytofluorimetry and detection of apoptotic cells before and after treatment with L-asparaginase were studied. Experiments have been conducted to investigate whether administration of L-asparaginase, which results in hydrolysis of extracellular asparagine, arrests asparagine dependent protein synthesis and causes subsequent inhibition of cell growth followed by decreased proliferation of leukemic cells.

Keywords: L-asparaginase, Tumor cells, Cytotoxicity, DNA and protein synthesis, Apoptosis.
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How to cite this article:

Sirisha, B., and Haritha, R. 2017. Efficacy of the Antiproliferative Effect of L-Asparaginase from Streptomyces enissocaesilis on Different Tumor Cell Lines.Int.J.Curr.Res.Aca.Rev. 5(7): 95-102
doi: https://doi.org/10.20546/ijcrar.2017.507.013