Abstract            Volume:5  Issue-8  Year-2017         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Low cost, efficient and reproducible protocol has been established through the technique of Plant tissue culture forced branch node for the propagation of an important bamboo species namely Bambusa vulgaris. The propagation of B. vulgaris through branch node culture was studied. Single node segments were tested for bud-break and shoot growth on basic Murashige and Skoog (1962) medium (MS) supplemented with different combinations and concentrations of growth regulators. Results suggest that cytokinin is important for bud-break. The position of the node on the Culm appears to affect bud-break and multiplication, middle nodes are the most suitable. Also, removal of prophylls enhances bud-break. High frequency multiple shoot induction was achieved from branch nodal segments collected from elite genotype on Murashige and Skoog’s (MS) medium supplemented with 1.5 mg/l 6-Benzylaminopurine (BAP) and 2.0 mg/l Kinetin (Kn). The size of explants and season greatly influenced the frequency of bud break. Best rooting response was observed on of Indole- 3 Butyric acid (IBA) 2mg/l with 60 % rooting. In vitro raised plants were successfully acclimatized and established in the field conditions where they exhibited normal growth.

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