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Abstract                 Volume:4  Issue-2  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Molecular Characterization of TEM, SHV and CTX M Genes among ESBL Producing Gram Negative Isolates in ICU
Vasumathi1, P.R. Thenmozhi Valli2, S. Senthamarai2*, C.P. Ramani3 and Raja Jagadeesan4
1Government Stanley Medical College, Chennai, Tamilnadu, India
2Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, Tamilnadu, India
3Institute of Microbiology, Madras Medical College, Tamilnadu, India
4Senior Assistant professor, King institute, Guindy, Tamilnadu, India
*Corresponding author
Abstract:

ESBL(extended spectrum β lactamase)  producing GNBs are continue to be the great challenge in clinical setups worldwide. The spread of ESBLs producing GNB has been rapidly increasing.T EM, SHV & CTX-M are the prevalent gene responsible for ESBL production. A total of 95 gram negative isolates were isolated from various clinical samples from ICU patients and subjected for ESBL screening and confirmed by combined disc diffusion method and MIC tests and 37 were ESBL producers. These strains were subjected for molecular analysis TEM, SHV and CTX-M genes. Among the 37 ESBL producers, CTX- M (48.3%) were the predominant gene of isolation in our study. SHV and TEM were 41.4 % and 17.2 % respectively. 68.7% of E.coli & 23.1% of Klebsiella spp had CTX-M genes, 92.3% of Klebsiella spp showed SHV genes and 12.5% E.coli & 23.1% of Klebsiella spp showed TEM genes. ESBL strains should be confirmed with the molecular analysis to know the prevalent gene and for epidemiological analysis.

Keywords: ESBL,TEM,SHV,CTX-M
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How to cite this article:

Vasumathi, A., P.R. Thenmozhi Valli, S. Senthamarai, C.P. Ramani and Raja Jagadeesan. 2016. Molecular Characterization of TEM, SHV and CTX M Genes among ESBL Producing Gram Negative Isolates in ICU.Int.J.Curr.Res.Aca.Rev. 4(2): 170-175
doi: http://dx.doi.org/10.20546/ijcrar.2016.402.021