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Abstract                 Volume:5  Issue-8  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Lipase Extracellular Activity from Non-Conventional Yeasts
Teresa Itandehui Garambullo-Peña, Yadira Carbajal Chávez and Juan Carlos González-Hernández*
Laboratorio de Bioquímica del Departamento de Ingeniería Química y Bioquímica del Instituto Tecnológico de Morelia, Av. Tecnológico # 1500, Colonia Lomas de Santiaguito, 58120, Morelia, Michoacán, México
*Corresponding author
Abstract:

The purpose of this study is to establish if the non-conventional yeasts Debaryomyces hansenii, Rhodotorula glutinis and Candida guilliermondii present lipase activity under saline stress growth conditions using NaCl and KCl, evaluating growing rate and adapting capacity to saline culture medium and enzymatic activity. A comparative analysis was done for the three yeasts in different growing conditions, with and without salt media in order to determine the best conditions for growing and enzymatic activity. This research pretends to look for a new biotechnological tool for the expanding applications in biotransformation area, such as the use of lipases in several industries, and a new source to obtain these enzymes. In order to do so, it is necessary to make an analysis of viability of extraction, and determine salt concentration in the incubation media as an important factor for growing, metabolism and enzymatic activity. D. hansenii ISA 1508 was the non-conventional strain with more enzymatic activity in saline stress condition at 30°C, using 0.6 M NaCl and 1.2 M NaCl, respectively. The p-nitrophenol method was used to measure lipase activity. The non-conventional yeast D. hansenii ISA 1508C showed tolerance to saline conditions and lipase activity. Yeasts C. guilliermondii, R. glutinis L-033 were found to have a similar enzymatic activity and did not show any preference for any of the experimental conditions probed herein.

Keywords: Lipases,Yeast,Enzyme.
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How to cite this article:

Teresa Itandehui Garambullo-Peña, Yadira Carbajal Chávez and Juan Carlos González-Hernández. 2017. Lipase Extracelular Activity from Non-Conventional Yeasts.Int.J.Curr.Res.Aca.Rev. 5(8): 75-80
doi: https://doi.org/10.20546/ijcrar.2017.508.011