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Abstract                 Volume:5  Issue-9  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Ligation, Transformation and Characterization of Rv 1984c Mycobacterium tuberculosis Indonesian Isolate as an Antigen for Latent TB Immunodiagnostic
Rosana Agus1*, Muhammad Nasrum Massi2 and Zaraswati Dwyana1
1Department of Biology, Faculty of Life Sciences, Hasanuddin University, Indonesia
2Department of Microbiology, Faculty of Medicine, Hasanuddin University, Indonesia
*Corresponding author
Abstract:

Latent tuberculosis infection (LTBI) is characterised by the presence of immune responses to Mycobacterium tuberculosis infection without clinical evidence of active Tuberculosis (TB). The challenge of identifying LTBI-infected individuals lies in the lack of a diagnostic gold standard for LTBI. Tuberculin skin test (TST) has been used for the diagnosis of tuberculosis for more than a century. Therefore TST has many limitations, so it is extremely urgent to develop a diagnose method to detect LTBI accurately. In this study we used, Culture Fitrate Protein 21 (CFP 21) encoded by the Rv1984c gene is an immunodominant protein and induces IFN-γ from TB patients. The purpose of this study was to ligate, transform and characterize the Rv1984 from M.tuberculosis isolates Indonesia to Escherichia coli JM109. The study was carried out by amplifying the Rv 1984c gene with PCR, ligation to pGEM-T vector and transformation to host cell Eschericia coli JM 109 on LB media induced by X-gal and IPTG. Characterization of pGEM-T-Rv 1984c recombinant clones was performed with PCR and sequencing. The results obtained that the DNA inserts that are ligated to the cloning vector is true Rv 1984 c

Keywords: Culture Fitrate Protein 21, Rv 1984c, Ligation, E. coli JM 109
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How to cite this article:

Rosana Agus, Muhammad Nasrum Massi and Zaraswati Dwyana. 2017. Ligation, Transformation and Characterization of Rv 1984c Mycobacterium tuberculosis Indonesian Isolate as an Antigen for Latent TB Immunodiagnostic.Int.J.Curr.Res.Aca.Rev. 5(9): 39-43
doi: https://dx.doi.org/10.20546/ijcrar.2017.509.006