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Abstract                 Volume:5  Issue-10  Year-2017          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Production and Optimization of Extracellular Polysaccharides from Paenibacillus sp.
D.S. Deepa Dhas1, Mohammed A. Almalki2, Ponnuswamy Vijayaraghavan1* and Rakesh Varghese3
1Bioprocess Engineering Division, Smykon Biotech Pvt Ltd, Nagercoil, Kanyakumari District, Tamil Nadu, India
2Biological Sciences Department, College of Science, King Faisal University, Saudi Arabia
3Department of Industrial Biotechnology, Bharath University, Selaiyur, Chennai 73, India
*Corresponding author
Abstract:

Extracellular polymeric substances (EPSs) are produced by a wide array of bacteria for protection against aggregation, expression of virulence and adhesion. Growth associated EPS production was determined by Paenibacillus sp. in submerged fermentation. To improve the productivity and efficiency of EPS from Paenibacillus sp. the physical factors and nutrient components were optimized. The bacterial growth (2.093 OD at 600 nm) was maximum after 36 h of incubation and EPS production (1.9 mg/ml) was high after 3 days of incubation at 37 °C. EPS production was found to be maximum at pH 7.0 (2.31 mg/ml), and at 40 °C (2.49 mg/ml). Supplementation of 1% sucrose (4.30 mg/ml) supported more EPS production. Among the nitrogen sources, addition of yeast extract (0.1%) supported maximum EPS production (5.36 mg/ml). Among inorganic sources, NH4Cl significantly influenced on EPS production.

Keywords: Extracellular polysaccharide, Paenibacillus sp. Optimization, Emulsifying agents
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How to cite this article:

Deepa Dhas, D.S., Mohammed A. Almalki, Ponnuswamy Vijayaraghavan and Rakesh Varghese. 2017. Production and Optimization of Extracellular Polysaccharides from Paenibacillus sp.Int.J.Curr.Res.Aca.Rev. 5(10): 1-7
doi: https://doi.org/10.20546/ijcrar.2017.510.001