Abstract Volume:5 Issue-12 Year-2017 Original Research Articles
|Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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2Department of pathology, Veterinary University, Vijayawada, Andhra Pradesh, India
Chicken Anaemia Virus (CAV) is an emerging disease of poultry especially in young chickens causing economic losses worldwide. High mortality in CAV infections were due to immunosuppression associated secondary bacterial and fungal infections. The disease was characterized by aplastic anaemia, haemorrhages in the muscle, thymus atrophy and immunosuppression. CAV infection is easily identified based on symptoms exhibited by diseased flocks. So the diagnosis of chicken anaemia virus is mainly based on external and clinical symptoms exhibited by infected chickens. Reduced haematocrit values were clearly observed in infected chicks after two weeks of post inoculation. Pathognomic signs and lesions exhibited by the affected flock. Tentatively the diagnosis can usually be made based on flock history, clinical signs, haematological changes and gross pathological findings in affected birds. PCR conformation is a specific diagnostic method used for conformation of virus by using quality primers to detect the CAV. For confirmatory diagnosis isolation and identification of the CAV is done. Reduced haematocrit (PCV) values are the sensitive indicator to identify clinically affected birds with CAV following experimental exposure. Monitoring of CIAV infection by virus isolation, cultivation along with application of molecular diagnostic tools such as polymerase chain reaction (PCR), etc. can be used for confirmatory diagnosis of CAV infection.
How to cite this article:Sandhya N., K. V. Subramanyam, D. V. R. Sai and Gopal D. V. R. 2017. Isolation, Cultivation and PCR Conformation of Chicken Anaemia Virus Isolates in Broiler Chicks of Coastal Region of Andhra Pradesh, India.Int.J.Curr.Res.Aca.Rev. 5(12): 40-45