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International Journal of Current Research and Academic Review-e-iSSN - 2347 - 3215

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Abstract                 Volume:5  Issue-12  Year-2017          Original Research Articles

Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Isolation, Cultivation and PCR Conformation of Chicken Anaemia Virus Isolates in Broiler Chicks of Coastal Region of Andhra Pradesh, India
Sandhya1, K. V. Subramanyam2, D. V. R. Sai1 and D. V. R. Gopal1*
1Department of Virology, S.V. University, Tirupati, Andhra Pradesh, India
2Department of pathology, Veterinary University, Vijayawada, Andhra Pradesh, India
*Corresponding author

Chicken Anaemia Virus (CAV) is an emerging disease of poultry especially in young chickens causing economic losses worldwide. High mortality in CAV infections were due to immunosuppression associated secondary bacterial and fungal infections. The disease was characterized by aplastic anaemia, haemorrhages in the muscle, thymus atrophy and immunosuppression. CAV infection is easily identified based on symptoms exhibited by diseased flocks. So the diagnosis of chicken anaemia virus is mainly based on external and clinical symptoms exhibited by infected chickens. Reduced haematocrit values were clearly observed in infected chicks after two weeks of post inoculation. Pathognomic signs and lesions exhibited by the affected flock. Tentatively the diagnosis can usually be made based on flock history, clinical signs, haematological changes and gross pathological findings in affected birds. PCR conformation is a specific diagnostic method used for conformation of virus by using quality primers to detect the CAV. For confirmatory diagnosis isolation and identification of the CAV is done. Reduced haematocrit (PCV) values are the sensitive indicator to identify clinically affected birds with CAV following experimental exposure. Monitoring of CIAV infection by virus isolation, cultivation along with application of molecular diagnostic tools such as polymerase chain reaction (PCR), etc. can be used for confirmatory diagnosis of CAV infection.

Keywords: Chicken,Diagnosis,Virus,Haematocrit value,Immunosuppression.
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How to cite this article:

Sandhya N., K. V. Subramanyam, D. V. R. Sai and Gopal D. V. R. 2017. Isolation, Cultivation and PCR Conformation of Chicken Anaemia Virus Isolates in Broiler Chicks of Coastal Region of Andhra Pradesh, India.Int.J.Curr.Res.Aca.Rev. 5(12): 40-45
doi: https://doi.org/10.20546/ijcrar.2017.512.006