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Abstract                 Volume:6  Issue-2  Year-2018          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Microbiological Quality and Physico-Chemical Composition of Domestic and Restaurant Wastewater as a Media for Microalgae Cultivation
K. Agwa*, K. F. Williams and B. N. Onwunali
Department of Microbiology, University of Port Harcourt, P M B 5323, Port Harcourt, Nigeria
*Corresponding author
Abstract:

Mass cultivation of algae has suffered series of set-backs and one of its challenges in media development. In this study, microbiological and physicochemical quality of the different domestic and restaurant wastewater were ascertained as potential feedstock for microalgae cultivation. The samples were prepared in 180:20, 160:40, 140:60, 120:80, and 100:100 ratios using pond water and mixture of domestic and restaurant waste water respectively. The blooming process was carried out under natural illumination for seven (7) days and the optical density (abs) and cell dry weight (mg/l) were monitored. The physico-chemical results showed that the wastewater were composed of vital inorganic nutrients such as nitrate (19.43±0.10, 17.48±0.56mg/l); sulphate (32.43±0.29, 33.38±0.21 mg/l); phosphate (3.27±0.12, 2.76±0.03 mg/l); ammonia (2.29±0.01, 2.15±0.03 mg/l); TDS (236.67±2.99, 236.33±2.91 mg/l); BOD (38.11±0.30, 39.66±0.70 mg/l); COD (283.95±0.25, 323.70±3.28 mg/l) for cultivation of microalgae. The total heterotrophic count for restaurant and domestic wastewater samples ranged from 1.06x106cfu/ml to 1.19x106cfu/ml and 1.0x104cfu/ml to 1.2x104cfu/ml for bacteria and fungi respectively. The bacterial isolates identified were Bacillus sp., Echerichia coli, Staphylococcus sp., Pseudomonas sp. and Salmonella sp. while fungal isolates were Penicillium sp., Aspergillus sp. and Mucor sp. The best optimal wavelength (620nm) was selected for growth monitoring at a ratio of 120:80 for restaurant: domestic wastewater. The inexpensive nutrient media composition of the wastewater could be positively harnessed in an integrated waste-microalgae mass cultivation process.

Keywords: Mass cultivation,Microalgae,Microbiological,Physicochemical,Wastewater.
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How to cite this article:

Agwa O. K., K. F. Williams and Onwunali B. N. 2018. Microbiological Quality and Physico-Chemical Composition of Domestic and Restaurant Wastewater as a Media for Microalgae Cultivation.Int.J.Curr.Res.Aca.Rev. 6(2): 62-70
doi: https://doi.org/10.20546/ijcrar.2018.602.007