IJCRAR is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRAR Articles.

INDEXED IN INDEX COPERNICUS - ICI Journals Master List 2016 - IJCRAR--ICV 2016: 81.15 For more details click here

Abstract                 Volume:6  Issue-10  Year-2018          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Efficacy of Some Laboratory Samples and Techniques in Detecting Chlamydia trachomatis Infection among Women in Kirkuk Province
Yahya Jirjees Salman1*, Eman Sabah Ahmed2 and Raghdaa Hussein Taqi2
1Microbiology and immunology department, Faculty of Medicine, Kirkuk University, Iraq
2Obestitric and gynecological department, Azadi Teaching Hospital-Kirkuk Health directorate, Iraq
*Corresponding author
Abstract:

Chlamydia trachomatis (CT), Trichomonas vaginalis, Neisseria gonorrheoae and other viruses are common causes for women urogenital discharges. The efficacy of laboratory methods and samples were aimed to reach final accurate, rapid method for diagnosis of CT.A total of 185 women urogenital samples tripled number from endocervical swabs, high vaginal swab(HVS) and urine deposits were collected from women in child bearing ages, involving 166 with urogenital discharges and the rest number as control (without discharge). For each specimen direct wet preparation, gram staining, rapid lateral immune-chromatography assay (RILCA) and ELISA technique were applied. The all rate of urogenital infection was 33, 23% Bacterial infection contributes high rate 55.22%, followed by CT 41.96%, fungi 22.23% and 12,58% for Trichomonas vaginalis. Gardnerella vaginalis and Moblincus species are dominant bacteria in HVS versus to CT in endocervical swabs. The rate of CT infection was high in specimen with yellowish discharge, RLICA technique is with efficacy to demonstrate CT than by using direct, gram staining, culturing and ELISA technique. The rate of CT was higher in the second and third period of gestation than in the first period. Young, aged women (15 to 30 years) are highly at risk for CT infection than elderly women. The relationship between women, abortion, parity and CT frequency was not significant. Pelvic inflammatory disease in high correlation to CT infection. Cervical erosion, vaginal itching, fever and dyspareunia are a dominant feature during women CT infection. Conclusions: The rate of STD was highest among young aged women particularly CT infection. Endocervical swabs and RLICA technique have had high efficacy in demonstrating CT than other lab methods. The relationship between genital discharge color gestational periods and CT existence was significant. Whereas it was negative in regard number of women abortions and women parity. This study was preliminary study that required further studies to a sure real rate of CT and other STD.

Keywords: Chlamydia; Trichomonas; Gardnerella; Endocervical; RLICA; ELISA
Download this article as Download

How to cite this article:

Yahya Jirjees Salman, Eman Sabah Ahmed and Raghdaa Hussein Taqi. 2018. Efficacy of Some Laboratory Samples and Techniques in Detecting Chlamydia trachomatis Infection among Women in Kirkuk Province.Int.J.Curr.Res.Aca.Rev. 6(10): 39-50
doi: https://doi.org/10.20546/ijcrar.2018.610.005