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Abstract                 Volume:4  Issue-2  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Standardization of Surface Sterilization Technique for In-vitro Propagation of Anthurium (Anthurium andraeanum Lind.) cv. Jewel
Rocky Thokchom* and Soumen Maitra
Department of Floriculture, Medicinal and Aromatic Plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar – 736165, West Bengal, India
*Corresponding author
Abstract:

An experiment was conducted to standardize the surface sterilization technique of Anthurium andraeanum cv. Jewel using immature bronze coloured leaves as explants for callus induction. Explants were collected, cleaned repeatedly using Tween–20 in fresh running water and then treated with 12 different combinations of Sodium hypochlorite (1.5%), Mercuric chloride (0.1%) and ethyl alcohol (70%) with varying exposure durations. Surface-sterilized explants were then inoculated in MS medium supplemented with various concentrations and combinations of 2,4-D, BAP and TDZ. Leaf explants when treated with mercuric chloride (0.1%) (3 minutes) + ethyl alcohol (70%) (30 seconds) resulted in lowest percentage (3.67) of contaminated cultures, lowest explant mortality (11.33%) as well as highest survivability (85.67%). Sodium hypochlorite (1.5%) (15 minutes) resulted in highest explant mortality (49.33%) while 5 minutes treatment resulted in lowest survivability (30.33%). Mercuric chloride (0.1%) (3 minutes) + ethyl alcohol (70%) (30 seconds) may be recommended for micropropagation of Anthurium through leaf explants.

Keywords: Anthurium, Contamination, explant,In vitro,Sterilization procedure
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How to cite this article:

Rocky Thokchom and Soumen Maitra. 2016. Standardization of Surface Sterilization Technique for In-vitro Propagation of Anthurium (Anthurium andraeanum Lind.) cv. Jewel.Int.J.Curr.Res.Aca.Rev. 4(2): 335-339
doi: http://dx.doi.org/10.20546/ijcrar.2016.402.036