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International Journal of Current Research and Academic Review-e-iSSN - 2347 - 3215

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Abstract                 Volume:7  Issue-5  Year-2019          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Evaluation of Staining Methods and Rapid Urease Test for the Detection of Helicobacter pylori in Gastric Biopsy Specimen among Patients with Dyspeptic Symptoms at a Tertiary Care Centre in Jammu
P. Sharma1, R. Tomar2* and S. Sudan1
1Government Medical College, Jammu, India
2SMC, Ghaziabad, India
*Corresponding author
Abstract:

In a developing country like India, Helicobacter pylori infection is a prevalent entity. It is an important human pathogen involved in the causation of pathogenesis of a number of diseases including Peptic Ulcer Disease (PUD) and Gastric Carcinoma. Diagnosis of Helicobacter pylori infection may be established by a number of Invasive and Non Invasive tests. In the present study we have evaluated two staining methods viz. Gram staining, Loeffler’s Methylene Blue Staining and Rapid Urease Test (RUT) for the detection of Helicobacter pylori in biopsy samples. Method: A total of 30 patients with gastric disorders (chronic gastritis and peptic ulcer disease) underwent upper gastrointestinal (GI) endoscopy with biopsy. Helicobacter pylori infection in gastric biopsies was identified after histopathological examination by microscopy and rapid urease test. H. pylori was detected in 14 (46.6%) out of 30 samples by histopathological method. 9 biopsy samples (30%) were positive by Gram Staining and 13 samples (43.3%) were positive by Loeffler’s Methylene Blue (LMB) staining. Of the total 30 samples, 11 samples (36.6%) were positive for Rapid Urease Test (RUT). 8 samples of the 9 samples positive in Gram Staining were also positive by RUT and all the 13 samples positive by LMB were also positive by RUT. Conclusion: LMB Staining method to stain and detect H. pylori is a better method than Gram Staining.

Keywords: Helicobacter pylori, Gastric biopsy, Rapid urease test, Staining methods.
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How to cite this article:

Sharma, P., R. Tomar and Sudan, S. 2019. Evaluation of Staining Methods and Rapid Urease Test for the Detection of Helicobacter pylori in Gastric Biopsy Specimen among Patients with Dyspeptic Symptoms at a Tertiary Care Centre in Jammu.Int.J.Curr.Res.Aca.Rev. 7(5): 59-64
doi: https://doi.org/10.20546/ijcrar.2019.705.008