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Abstract                 Volume:7  Issue-6  Year-2019          Original Research Articles


Online ISSN : 2347 - 3215
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Isolation and Characterization of Pox Virus Circulating in Sheep and Goats from Outbreak Cases of Adea Berga District, West Shoa Zone, Oromia, Ethiopia
Abdi Assefa1, Barbara Weilnda2 and Fufa Abunna3*
1Adea Berga Livestock and Fishery Development, Enchini, Ethiopia
2The International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia
3Addis Ababa University, CVCMA, P.O. Box, 34, Bishoftu, Ethiopia
*Corresponding author
Abstract:

A cross-sectional study was conducted to isolate and characterize the pox viruses circulating in sheep and goats from outbreak cases of Adea Berga district from November 2015 to March 2017 using tissue culture and PCR. The study was employed questionnaire survey, outbreak investigation, virus isolation and molecular characterization. A total of 600 sheep and goats (412 sheep and 188 goats) from ten peasant associations (in which the outbreaks were occurred) were clinically examined for the presence of pox lesions on their skin. Out of these, 137(33.3%) sheep and 51(27%) goats had pox lesions on their skin. The questionnaire survey indicated that sheep and goat pox was the most common disease and frequently observed during the long rainy (Ganna) and short rainy (Afrasa) seasons. The overall morbidity and mortality proportion of sheep and goat pox was 31.3% and 4.5%, respectively. High mortality rate was observed in young age groups with odd ratio 1.90 at 95% CI (p < 0.05). From 27 tissue sample collected, the virus was isolated from 25 skin samples (13 sheep and 12 goats). The tissue culture showed a typical characteristic of pox virus: cytopathic effect of cell syncytia, ballooning, aggregation and detaching of cells on Vero cell culture. Similarly, the conventional PCR revealed that 25 out of 27 tested samples were positive by developing band size of 172bp (goat pox virus) whereas two of them could not produce any band size on gel electrophoresis. Even though the existing knowledge suggested that Capri pox virus is strictly host specific the PCR result confirmed that sheep were affected by goat pox virus similarly to goats and hence classification of pox virus based on infected host in small ruminant has been found to be inconclusive. Thus, genotyping of the isolates should be conducted carefully instead of naming the virus genotype based on the name of animals from which the samples have been collected.

Keywords: Adea Berga; Sheep and Goat; Pox virus; Outbreak; PCR
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How to cite this article:

Abdi Assefa, Barbara Weilnda and Fufa Abunna. 2019. Isolation and Characterization of Pox Virus Circulating in Sheep and Goats from Outbreak Cases of Adea Berga District, West Shoa Zone, Oromia, Ethiopia.Int.J.Curr.Res.Aca.Rev. 7(6): 1-21
doi: https://doi.org/10.20546/ijcrar.2019.706.001