Abstract Volume:4 Issue-1 Year-2016 Original Research Articles
|Online ISSN : 2347 - 3215
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Department of Biology, College of Education, University of Salahadin, Erbil, Iraq
Present investigation includes the quantitative estimation of protein, carbohydrate, lipids and nucleic acids in fish intestinal helminthes, Khawia armeniaca, Bothriocephalus acheilognathi, Senga sp., Postgangesia inarmata, Procamallanus viviparous, Neoechinorhynchus iraqensis, and Neoechinorhynchus zabensis. In addition, these helminthes were characterized biochemically using electrophoretic isoenzyme patterns and genetically using a molecular approach. The results revealed that the protein and glycogen contents were low in parasites as compared to their hosts. In contrast,lipids were high in parasites as compared to their hosts.DNA was extracted from helminthes and used as template to amplify 5.8 S ribosomal DNA primers and analysis of PCR product revealed positive band of 450 pb in acanthocephalans. Isoenzyme electrophoresis revealed the presence of two bands for each hexokinase and malic enzyme in acanthocephalans and one band in cestodes whereas malate dehydrogenase was with two bands in cestodes and with one band in acanthocephalans.
How to cite this article:Husain F. Hassan, Dalya S.Hashim and Shamall M.A. Abdullah. 2016. Identification of Some Iraqi Fish Parasites by using Biochemical and Molecular Protocols.Int.J.Curr.Res.Aca.Rev. 4(1): 54-64