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Abstract                 Volume:4  Issue-1  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Identification of Some Iraqi Fish Parasites by using Biochemical and Molecular Protocols
Husain F. Hassan1 *, Dalya S.Hashim1 and Shamall M.A. Abdullah2
Department of Biology, College of Science, University of Kirkuk, Iraq
Department of Biology, College of Education, University of Salahadin, Erbil, Iraq
*Corresponding author
Abstract:

Present investigation includes the quantitative estimation of protein, carbohydrate, lipids and nucleic acids in fish intestinal helminthes, Khawia armeniaca, Bothriocephalus acheilognathi, Senga sp., Postgangesia inarmata, Procamallanus viviparous, Neoechinorhynchus iraqensis, and Neoechinorhynchus zabensis. In addition, these helminthes were characterized biochemically using electrophoretic isoenzyme patterns and genetically using a molecular approach. The results revealed that the protein and glycogen contents were low in parasites as compared to their hosts. In contrast,lipids were high in parasites as compared to their hosts.DNA was extracted from helminthes and used as template to amplify 5.8 S ribosomal DNA primers and analysis of PCR product revealed positive band of 450 pb in acanthocephalans. Isoenzyme electrophoresis revealed the presence of two bands for each hexokinase and malic enzyme in acanthocephalans and one band in cestodes whereas malate dehydrogenase was with two bands in cestodes and with one band in acanthocephalans.

Keywords: Protein, Glycogen, Lipid, DNA, Cestode, Acanthocephalans, Nematode, marine
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How to cite this article:

Husain F. Hassan, Dalya S.Hashim and Shamall M.A. Abdullah. 2016. Identification of Some Iraqi Fish Parasites by using Biochemical and Molecular Protocols.Int.J.Curr.Res.Aca.Rev. 4(1): 54-64
doi: http://dx.doi.org/10.20546/ijcrar.2016.401.006