Abstract            Volume:7  Issue-6  Year-2019         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Proteases are the important compounds of all life on earth including fungi, plants, animals and prokaryotes. About 60% of the total enzyme market has been grabbed by protease. Protease may also be called as proteinase, peptidases or proteolytic enzyme that hydrolysis (Addition of water across the peptide bonds) the peptide bond in between the amino acids. Depending on their acid base behavior they are divided into 3 groups acid protease produced by mostly fungi, Neutral protease produced by plants sources and finally Alkaline protease. Bacillus species are well known for their extracellular protease enzyme production and industries majorly uses Bacillus subtilis for the production of various enzymes. Bacillus is rod shaped bacterium with protective endospore that can withstand extreme conditions. They are obligate aerobes or facultative anaerobes in which few are pathogenic and others are free living. The present study reports The Screening and characterization of proteolytic bacteria from various samples and its application in antibiofilm activity. 34 bacterial species were isolated and screened. Two bacterial species (LSSP03 and SCPW01) were selected for the optimization of protease enzyme using various parameters such as carbon, nitrogen and pH. In order to identify the bacteria species using molecular methods two isolates (FS 1 & SP1) were selected for genome sequencing. The protease produced from the bacteria was checked for its antibacterial activity using well diffusion method and the antibiofilm activity was checked against the biofilm produced by Pseudomonas aeruginosa by Tube and Microtitre plate method.

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