Abstract            Volume:7  Issue-6  Year-2019         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Shrimp are swimming, decapods crustaceans classified in the order Cardiea, found widely around the world in both fresh and salt water. Asia has always led world production of cultivated shrimp with a market value of billions of US dollars per year. Shrimp aquaculture is one of the fastest growing agricultural industries, with more than 10% average growth in last decade. The extensive farming of shrimp in ponds has widely increases the chitinolytic microorganisms due to the abundance nature of chitinous wastes. Bacillus species are well known for their extracellular chitinase enzyme production and industries majorly uses Bacillus subtilis for the production of various enzymes. Bacillus is rod shaped bacterium with protective endospore that can withstand extreme conditions. They are obligate aerobes or facultative anaerobes in which few are pathogenic and others are free living. The present study reports The Screening and characterization of chitinolytic bacteria from various shrimp ponds. From 11 different samples 40 bacterial species were isolated and screened. Two bacterial species KK2 and VMP were selected for the optimization of chitinase enzyme using various parameters such as carbon, nitrogen and pH. In order to identify the bacteria species using molecular methods two isolates KK2 and VMP were selected for genome sequencing.

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