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Abstract                 Volume:8  Issue-1  Year-2020          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Isolation, Identification and Characterization Lactic Acid Bacteria from Raw Cow Milk, Soil and Wastes
Fikiru Getachew1* and Sara Endale2
1Salale University, Department of Animal
2Dilla University, Department of Animal and Range Science, P. O, Box419, Dilla; Ethiopia
*Corresponding author
Abstract:

Twenty(20) isolates of lactic acid bacteria were selected from all samples (Milk, Dung, Sewage, wastes, and soil). Bacteria loads from each sample were ranging from 5x103cfu/ml to 7.4x108cfu/ml especially higher in dung samples. Each isolate was characterized physiologically and biochemically based on growth temperature, pH and fermentation ability on different sugars and the suitability of isolate was tested based on a clear zone formed on skim milk medium. The dominant species found were Lactococcus lactis (30%), Lactobacillus brevis (20%), Streptococcus thermophiles (15%), Enterococcus feacalis (15%), Lactobacillus lactis (10%) and Lactobacillus delbrueki (10%). All most all isolates are suitable for starter culture and can be used in dairy food and feed industries. Thus, further characterization to the molecular level, treatment of feed with these isolates and evaluation of their capacity is needed further investigations.

Keywords: Lactic acid bacteria, Starter Culture, Wastes
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How to cite this article:

Fikiru Getachew and Sara Endale. 2020. Isolation, Identification and Characterization Lactic Acid Bacteria from Raw Cow Milk, Soil and Wastes.Int.J.Curr.Res.Aca.Rev. 8(1): 36-46
doi: https://doi.org/10.20546/ijcrar.2020.801.006