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Abstract            Volume:8  Issue-7  Year-2020         Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Phytochemical Screening and Antimicrobial Activities of Purslane Leaf and Stem Extracts
Mulugeta Desta, Kindu Agegnew and Zekeria Yusuf
College of Natural & Computational Sciences School of Biological Sciences and Biotechnology, Haramaya University, Ethiopia
*Corresponding author
Abstract:

In spite of the fact that pharmaceutical companies have annually produced a huge quantity of antibiotics, emerging bacterial resistance is a main concern for the medical field. Ethno pharmacological studies show that plants and their products are a good source of biologically active antibacterial agents. Characterization of the phytochemical and antimicrobial activity from leaves and steam of purslane and appraisal of its viability is the main purpose of this study. In this work, the phytochemical and antimicrobial activity from leaves and steam of purslane, were determined. The methanol extracted samples from leaf and stem of purslane were made at three concentration levels. Antimicrobial activities were determined using disc diffusion method. Methanol extracts of leaf and stem revealed the presence of tannins, saponins, steroids, terpenoids, and alkaloids as the active phytoconstituents of purslane. The strongest antibacterial activity with maximum zone of inhibition (16.8mm) was recorded with methanol leaf extract against S. aureus at 200 mg/mL of concentration. Minimum inhibitory concentration and minimum bactericidal concentration values of 10 mg/mL and 20mg/mL respectively against S. aureus; and 100 mg/ml and 125mg/ml respectively against Aspergillus niger. These results will facilitate detailed phytochemical and antimicrobial activity purslane further elucidation of its biochemical function.

Keywords: Antibacterial, Disc diffusion, Methanol, Pathogenic, Phytochemical
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How to cite this article:

Mulugeta Desta, Kindu Agegnew and Zekeria Yusuf. 2020. Phytochemical Screening and Antimicrobial Activities of Purslane Leaf and Stem Extracts.Int.J.Curr.Res.Aca.Rev. 8(7): 88-93
doi: https://doi.org/10.20546/ijcrar.2020.807.010
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.