Abstract            Volume:8  Issue-11  Year-2020         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

The capacity of indigenous rhizobia to nodulate a specific legume crop effectively, or to act as barrier to the successful establishment of inoculant strains, is critical to successful establishment and growth of legumes in the respected agroecological zone. Effective groups serve both as a guide to inoculant preparation and as a basis for predicting the need for inoculation in future perspective. This review included all aspects of rhizobial work basic protocols, starting from isolation and characterization of root nodule rhizobia from the laboratory to authentication of the symbiotic effectiveness test under sterile sand condition in the greenhouse. This review shows the process on how to isolate, characterize and evaluate the symbiotic effectiveness along with growth responses to varied physiological conditions of pH, temperature, antibiotics, heavy metals as well as wide range of carbon and nitrogen sources. The main goal of repeated works in this regard is to discover new and better strains for use in legume inoculants so as to improve agricultural productivitiy. This pursuit entails the collection of isolates, characterization, assessment of symbiotic capacity and comparison to strains currently included within inoculants. This was a very valuable review for researchers not only for accomplishing initial steps for getting new strains but dealing with improving soil fertility and supporting sustainable crop production with the minimum use of costly and environmentally unfavorable chemical fertilizers.

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