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Abstract                 Volume:8  Issue-11  Year-2020          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Characterization of the fibrinolytic enzymes producing bacterium strain (isolate SWN 55) from the soil waste
Sateesh Waradeva*
Siddagagnga College for Arts, Science and Commerce, Tumakuru, Karnataka, India
*Corresponding author
Abstract:

The present work was conducted to characterize the  fibrinolytic enzyme produced by the strains of the isolates of  Streptomyces sp. obtained from the soil waste. The isolated organism was cultured in the sterile media at 37ºC for 24 hrs. The selected isolates were subjected to fibrinolytic activitiy. The fibrinolytic activity of isolates SWN 55 was higher and it was subjected for biochemical studies. Based on the study, isolate was designated as Streptomyces aureus SWN 55. Further it was subjected to three step purification involving ammonium sulphate precipitation, Sephadex G-50 gel permeation chromatography and DEAE Sephadex A-50 anion exchange chromatography. After the purification processes, the molecular weight of fibrinolytic protease enzyme is 31kDa.

Keywords: Fibrinolytic activity, purification, ammonium sulphate precipitation, Sephadex G-50 gel permeation chromatography and DEAE Sephadex A-50 anion exchange chromatography
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How to cite this article:

Sateesh Waradeva, N. 2020. Characterization of the fibrinolytic enzymes producing bacterium strain (isolate SWN 55) from the soil waste.Int.J.Curr.Res.Aca.Rev. 8(11): 97-105
doi: https://doi.org/10.20546/ijcrar.2020.811.011