Abstract            Volume:10  Issue-4  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l mg/l indol -3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23 ± 0.21) with a maximum shoot length7.68 ± 0.24cm and 6.79 ± 0.09 shoot number with 7.47± 0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03 ± 0.49 and root length10.76± 0.16 cm and 10.58 ± 0.26 root number with 10.42 ± 0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.

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