Abstract            Volume:10  Issue-5  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Maize is used for human and animal consumption, as a biofuel, and for a range of industrial purposes around the globe. In modern agriculture, farmers choose between two types of maize varieties hybrids and open-pollinated varieties and their choice depends primarily on the prevalent environmental and economic situation as well as the availability of seed of the preferred variety type. Therefore to supply improved varieties reducing the time needed for inbred development is the key role. This can be most effectively achieved by application of the doubled haploid (DH) technology. The objective of this topic is to review the method and application of DH technology in maize breeding. Producing DH lines typically requires four steps: inducing haploids by crossing heterozygous plants with a haploid inducer; identifying haploid kernels through morphological markers; chromosome doubling of haploids by colchicine treatment; and selfing to obtain seeds of DH lines. The utility of DHs in homozygous lines development, recurrent selection, induction of mutation, genetic enrichment, and integrity with other technologies make them the best material for genetic improvement and genetic studies of maize.

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