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Abstract                 Volume:4  Issue-4  Year-2016          Original Research Articles


Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
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Isolation of L-asparaginase Producing Actinomycetes from Marine Sediments
Dhevagi1* and E. Poorani2
1Department of Environmental Sciences, Tamil Nadu Agricultural University,Coimbatore – 641 003, Tamil Nadu, India
2Department of Biotechnology, Government College of Technology, Coimbatore – 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

Environmental factors in the marine environment forces the marine organisms to be the source of useful bioactive compounds. Among the marine organisms, Actinomycetes gained importance as they play a vital role in the production novel metabolites. To explore the potential of marine Actinomycetes the present study was carried out. Eight isolates with different growth pattern was isolated from marine sediments of Thiruvallum, Kerala using starch casein agar medium. Large size, white colored colonies with fibre like growth surrounding the colonies were observed in starch casein agar media. All the isolates were subjected to rapid plate assay technique for screening L asparaginase activity. Starch casein agar supplemented with 0.015 % phenol red was highly suitable for assessing asparaginase activity. Only three isolates showed significant activities and KTI7 showed highest zone of clearance. The crude enzyme obtained from the promising isolate KTI7 was purified by salting out with ammonium sulphate followed by sephadex gel filtration.

Keywords: L-asparaginase,Actinomycetes,Marine Sediments,Ammonium Sulphate.
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How to cite this article:

Dhevagi, P., and Poorani, E. 2016. Isolation of L-asparaginase Producing Actinomycetes from Marine Sediments.Int.J.Curr.Res.Aca.Rev. 4(4): 88-97
doi: http://dx.doi.org/10.20546/ijcrar.2016.404.011