Abstract            Volume:10  Issue-12  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Phosphatases are crucial in solubilization of phosphorus. Microorganisms producing phosphatases transform plant unavailable phosphorus into available forms. The enzymes have wide range of applications in laboratories and industries. The phosphatases producing bacteria were screened on nutrient phosphate agar whereas fungi on potato dextrose phosphate agar medium. The detection plates were flooded with Gram’s iodine solution after incubation at the respective temperatures. Clear hydrolyzed zones were developed around the colonies indicating the phosphatase production. The present developed method is simple and rapid for screening of both bacteria and fungi that produce extracellular phophatases. Promising isolates producing phosphatases can be detected by measuring the hydrolyzed zones on agar media. The levels of phosphate solubilization efficiency and alkaline phosphatase production capabilities were gradually increased indicating growth associated pattern. In the present study a novel, sensitive and efficient plate assay method was developed for screening of potential microorganisms (both bacteria and fungi) producing phosphatases.

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