IJCRAR is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRAR Articles.

INDEXED IN INDEX COPERNICUS - ICI Journals Master List 2016 - IJCRAR--ICV 2016: 81.15 For more details click here

Abstract            Volume:10  Issue-12  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

Novel, Rapid and Sensitive Plate Assay Method for Detection and Screening of Phosphatase Producing Microorganisms
P. Nalini* and R. S. Prakasham
Medicinal Chemistry and Biotechnology Lab-Organic Synthesis and Process Chemistry Division,CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad - 500007, Telangana, India.
*Corresponding author

Phosphatases are crucial in solubilization of phosphorus. Microorganisms producing phosphatases transform plant unavailable phosphorus into available forms. The enzymes have wide range of applications in laboratories and industries. The phosphatases producing bacteria were screened on nutrient phosphate agar whereas fungi on potato dextrose phosphate agar medium. The detection plates were flooded with Gram’s iodine solution after incubation at the respective temperatures. Clear hydrolyzed zones were developed around the colonies indicating the phosphatase production. The present developed method is simple and rapid for screening of both bacteria and fungi that produce extracellular phophatases. Promising isolates producing phosphatases can be detected by measuring the hydrolyzed zones on agar media. The levels of phosphate solubilization efficiency and alkaline phosphatase production capabilities were gradually increased indicating growth associated pattern. In the present study a novel, sensitive and efficient plate assay method was developed for screening of potential microorganisms (both bacteria and fungi) producing phosphatases.

Keywords: Microorganisms, screening, phosphatase, Gram’s iodine
Download this article as Download

How to cite this article:

Nalini, P. and Prakasham, R. S. 2022. Novel, Rapid and Sensitive Plate Assay Method for Detection and Screening of Phosphatase Producing Microorganisms.Int.J.Curr.Res.Aca.Rev. 10(12): 1-11
doi: https://doi.org/10.20546/ijcrar.2022.1012.001
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.