International Journal of Current Research and Academic Review
ISSN:2347 - 3215 Volume 1 Number 1 (2013) pp. 17-25
Isolation, molecular characterization and antibiogram of bacteria isolated
from diabetic foot ulcers
T. Mathangi1*, P. Prabhakaran2, Flora Rayappan3 and Florida Tilton3
1Research Scholar, Department of Microbiology, PRIST University, Thanjavur, India
2Professor, Department of Microbiology, Shrimad Aandavar College, Thiruchirappalli, India
3Biozone Research Technologies Pvt. Ltd., Chennai, India
Diabetic patients have increased risk of foot ulcers leading to minor or major
amputation. This is habitually caused either by neuropathy or ischaemia, in
few cases by both. Initial cause for such ulcers may be due to acute
mechanical or thermal trauma or by repeated mechanical stress. Multidrug
resistant aerobes have been closely related to diabetic foot infections. A high
percentage of Staphylococcus aureus isolates were reported in hospitals
associated with ulcer infections (Sanjay et al., 2013). In this study, swab
samples from diabetic patients foot ulcers from hospitals in and around
Chennai were collected. Out of 22 isolates, 4 strains showed high Multiple
Antibiotic Resistant (MAR) index. 16S rRNA gene was amplified using PCR
technique in all the 4 strains and they were characterized as Staphylococcus
aureus, Morganella morganii, Acinetobacter baumannii and Acinetobacter
Spp. Phylogenetic tree for each of the isolate was constructed to analyze its
evolutionary relationship with closely related species.
Diabetic foot ulcers;
aureus; Multiple
antibiotic resistance;
Phylogenetic tree