| Lipase production was carried by Serratia marcescens. Studies were undertaken to improve lipase production by optimizing pH, incubation time, and temperature. The effect of carbon source was studied by adding glucose, fructose, sucrose, lactose, mannitol, glycerol, starch, groundnut meal and soya
meal to a medium containing nitrate and other mineral source. The best activity of 6.102 U/ml after 45 hour at 30 o C at pH 7 was obtained in the media supplemented with glucose and starch. Peptone, soyatone, tryptone, casein, yeast extract, beef extract, ammonium nitrate, ammonium chloride, ammonium sulphate and sodium nitrate were tested as nitrogen source in this medium, with casein giving a lipolytic activity of 8.441 U/ml after 45 hour, the highest yield obtained in this study. Lipolytic activity yield was high in gingly oil about 8.595 U/ml at pH 7 after 45 hour incubation than other substrate source. Incubation time, pH and temperature of the medium were same in all the case. |