International Journal of Current Research and Academic Review

Int.J.Curr.Res.Aca.Rev. 2014.2(1): 41-45

Detection and Identification of Seed Mycoflora of Safflower

D.Amrutha Gayathri1* , V.Krishna Rao1 , B.Rajeswari1 and T.Ramesh Babu2

1Department of Plant Pathology, College of Agriculture, Acharya N G Ranga Agricultural University, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India 2Department of Entomology, College of Agriculture, Acharya N G Ranga Agricultural University, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India

A B S T R A C T

An investigation was conducted to detect the associated seed mycoflora in safflower and its control. A total of 19 safflower seed samples were collected from major growing areas of safflower. Blotter method and agar plate methods were used for detection of seed mycoflora of safflower seeds. Across the two methods adopted, a total of seven fungal genera including Alternaria, Aspergillus, Chaetomium, Rhizopus, Curvularia and Fusarium were detected. The fungi detected were identified based on their cultural and morphological characteristics.The fungal species namely Alternaria carthami, Aspergillus niger was found associated with all the tested hybrids/varieties/germplasms while species of Chaetomium sp, Rhizopus sp, Curvularia sp and Fusarium sp were not detected in some of the hybrids/varieties/germplasms. Among the seven fungal species detected the occurrence of Alternaria carthami was high 47.94 per cent followed by Aspergillus niger. The cultivar, Nira showed higher per cent incidence of seed mycoflora. Per cent incidence of seed mycoflora varied across the methods adopted and cultivars tested. The highest per cent incidence of 46% was observed with the fungus Alternaria carthami on Nari NH1 in blotter method. Out of the two methods tested blotter method was found superior over agar plate method in which higher number of fungi were recorded.

Keywords

Safflower; Mycoflora; Alternaria carthami; seed borne