Abstract            Volume:13  Issue-9  Year-2025         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Plectranthus edulis is a highly important tuber crop due to its medicinal and nutritional value. However, its production has been threatened due to the insufficiency of good propagation material. Therefore, a study was conducted to develop a protocol for the Indirect in vitro regeneration of P.edulis from shoot tip and internode explants. The results revealed that surface sterilization with 0.5 and 1% NaOCl for 10 minutes was effective for disinfecting shoot tip and internode explants, respectively. In callus induction, the highest callus induction percentages (100% and 93.75±6.25%) were obtained from 1.5 and 2mg/l 2, 4-D that supplemented MS media for shoot tip and internode explants, respectively. The highest percentage of shoot regeneration was achieved (66.67±8.33%) for shoot tip and internode explants cultured on MS medium with 0.8 and 1mg/l BAP. The highest multiplication rate (9.89) in the multiplication stage was obtained from MS medium supplemented with 1.5mg/l BAP. High rate (100%) root induction and root number (9.34) were achieved on half MS medium supplemented with 1.5mg/l and 2mg/l IBA, respectively. Acclimatization of plantlets with river sand and forest soil with 1:2 ratios gave 57.14% survival for both explants. The results of the study demonstrated a successful protocol for micro-propagation of P.edulis (212464/1984) through indirect organogenesis that can be used to address its production constraints. To determine its reproducibility and further enhance the efficiency of laboratory-based propagation protocol, it is essential to incorporate additional accessions and various combinations of PGRs.

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